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Registros recuperados: 25 | |
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Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M.. |
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602 |
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Chang,Ermei; Zhao,Yuxin; Wei,Qianchun; Shi,Shengqing; Jiang,Zeping. |
Platycladus orientalis has a lifespan of several thousand years in China, making it a good plant in which to study aging at the molecular level, but this requires sufficient quantities of high-quality P. orientalis RNA. However, no appropriate methods have been reported for total RNA isolation from P. orientalis leaves. The TRIzol method did not extract RNA, while cetyltrimethylammonium bromide, sodium dodecyl sulfate-phenol, and plant RNAout kit (Tianz, Inc., China) protocols resulted in low yields of poor quality RNA. Isolating total RNA using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) resulted in a high-quality product but a low yield. However, the two-step removal of polyphenols and polysaccharides in the improved plant... |
Tipo: Journal article |
Palavras-chave: Cupressaceae plants; Polysaccharides; Polyphenols; Total RNA isolation; Two-step removal of polyphenols and polysaccharides. |
Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500004 |
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García Ruiz, Mayra Teresa. |
Se evaluaron los cambios de acidez titulable a lo largo de 24 h, de las yemas vegetativas con longitud media de 0.5 cm y nopalitos de 6 y 11 cm, de Opuntia ficus-indica de los cultivares Atlixco, Copena V1, Milpa Alta, Moradaza y Solferino, con riego. También se evaluó el efecto de la disminución del potencial de agua (ΨA) del suelo, por suspensión del riego durante 60 días, en condiciones de invernadero, en el potencial osmótico (ΨS ), liberación de electrolitos (o índice de daño membranal, Id) y contenido de polisacáridos estructurales de los nopalitos de esos cinco cultivares y una recolecta silvestre de O. streptacantha. La acidez de las yemas vegetativas y los nopalitos fluctuó entre 0.2 y 0.9 % a lo largo de las 24 horas del día, con las... |
Tipo: Tesis |
Palavras-chave: Acidez titulable; Potencial hídrico; Potencial osmótico; Polisacáridos; Maestría; Fisiología Vegetal; Tritable acidity; Water potential; Osmotic potential; Polysaccharides. |
Ano: 2007 |
URL: http://hdl.handle.net/10521/1654 |
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Gil Cortiella,Mariona; Peña-Neira,Álvaro. |
Obtaining polysaccharides from grape cell walls is an interesting topic for the wine industry given its implications with regards to wine treatments and potential sensory attributes. In the present work, several conditions (i.e., a chelating agent, pH, titratable acidity, extraction time, number of successive extractions, solid to liquid ratio and alcoholic fermentation) were tested to assess their effects on the yield of soluble polysaccharides obtained from grape skins. Among the four tested solvents (i.e., water, tartaric acid, ammonium oxalate, and ethylenediaminetetraacetic acid), aqueous ammonium oxalate was the most effective for polysaccharide extraction but also produced the highest amount of protein impurities. Increasing the number of successive... |
Tipo: Journal article |
Palavras-chave: Carménère; Extraction; Grape skin polysaccharides; Pectin; Polysaccharides; Wine. |
Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000100009 |
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Lebellenger, Lou. |
The marine bacteria Vibrio diabolicus produces an exopolysaccharide (EPS) called HE800. The native form of HE800 has already shown biological efficiency in bone healing. It is studied as glycosaminoglycan-mimetic for applications in cosmetic and human health. To enhance biotechnological applications of polysaccharides, the chemical structures should be properly controlled; moreover, the production yield could also be a bottleneck. The genetic cluster of the HE800 EPS biosynthesis has been identified in 2014. However, the biosynthesis molecular mechanisms and regulations remain sometimes unclear. The main objective of this work is to obtain a recombinant production of the HE800 EPS in the common host Escherichia coli, to improve the knowledge of its... |
Tipo: Text |
Palavras-chave: Vibrio diabolicus; Polysaccharides; HE800; GAGmimétiques; Biosynthèse; Vibrio diabolicus; Polysaccharides; HE800; GAGmimetics; Biosynthesis.. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00497/60911/64261.pdf |
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MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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Merceron, Christophe; Portron, Sophie; Vignes-colombeix, Caroline; Rederstorff, Emilie; Masson, Martial; Lesoeur, Julie; Sourice, Sophie; Sinquin, Corinne; Colliec-jouault, Sylvia; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome. |
Mesenchymal stem cells (MSCs) are considered as an attractive source of cells for cartilage engineering due to their availability and capacity for expansion and multipotency. Differentiation of MSC into chondrocytes is crucial to successful cartilage regeneration and can be induced by various biological agents, including polysaccharides that participate in many biological processes through interactions with growth factors. Here, we hypothesize that growth factor-induced differentiation of MSC can be increased by chemically oversulfated marine polysaccharides. To test our hypothesis, human adipose tissue-derived MSCs (hATSCs) were cultured in pellets with transforming growth factor (TGF)-beta 1-supplemented chondrogenic medium containing either the... |
Tipo: Text |
Palavras-chave: Cartilage; Adipose-derived mesenchymal stem cells; Polysaccharides; Transforming growth factor-beta. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00071/18227/17457.pdf |
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Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia. |
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared... |
Tipo: Text |
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf |
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Rubio-Piña,Jorge A; Zapata-Pérez,Omar. |
RNA extraction from mangrove tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds upon cell disruption. Besides, a successful RNA isolation from mangrove tissues sometimes can be strain and species specific. Two different methods were used to extract RNA from tissues (stems, leaves and roots) of black mangrove (Avicennia germinans) and white mangrove (Laguncularia racemosa), collected from the mangrove area at Progreso, Yucatan, Mexico. An optimized and modified total RNA isolation method was developed for these recalcitrant species. The protocol is based on the CTAB method including β-mercaptoethanol and PVPP with sequential Phenol/Chloroform/isoamyl alcohol extractions to remove protein, and polyphenols,... |
Tipo: Journal article |
Palavras-chave: Mangrove; Plant tissues; Polysaccharides; RNA isolation. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500011 |
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Registros recuperados: 25 | |
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